I can provide some general insights into feline behavior and cognition.
Cats are perceptive animals with keen senses, and they can notice changes in their environment. If you've cleaned your room and made significant changes to the layout or removed familiar scents, your cat is likely to notice the difference. Cats rely heavily on their sense of smell, so if you've used cleaning agents or rearranged items with distinct scents, your cat might be able to detect those changes.
Whether your cat "knows" that you've specifically cleaned the room is challenging to determine definitively. Cats don't have the same level of complex cognitive abilities as humans, so they may not comprehend the concept of cleaning as we do. Instead, they'll respond to the changes they perceive using their senses.
Your cat's reaction to the cleaned room could vary. Some cats may be curious about the changes and explore the room more than usual, while others might become wary or stressed due to the altered environment. Cats are creatures of habit, and abrupt changes can sometimes be unsettling for them.
In any case, if your cat seems agitated or uneasy after you've cleaned the room, provide them with some extra attention and reassurance. Over time, they should adjust to the changes and return to their usual behaviors. Always observe your cat's reactions to understand how they are responding to changes in their surroundings.